Figure 3.

rVISTA analysis algorithm identifies experimentally characterized TFBS. (A) Two functionally characterized NFAT/AP-1 clusters indicated by black vertical arrows ([two sites/60 bp] [Table 1: E7 and E8]) are identified by rVISTA and are the only two clusters of conserved TFBSs present in the IL-5 promoter. The VISTA alignment highlights exons in blue, UTRs in yellow, and conserved noncoding in red. (B) A GATA-3 pair in the IL-5 promoter indicated by black vertical arrow is highly conserved and represents the only functional GATA-3 cluster ([2 GATA/60 bp] [Table 1]) in the proximal promoter (500 bp upstream of the 5′UTR) of this cytokine.