TABLE 2.
Oligonucleotide primers used in PCR assays
| PCR | Primer | Sequence (5′-3′) | Target gene | PCR product (bp) | Annealing temp (°C) | No. of cycles |
|---|---|---|---|---|---|---|
| A | B4 (8) | TGGCTCGGTTGCCAATATCAA | bcsp31a | 223 | 60 | 40 |
| B5 (8) | CGCGCTTGCCTTTCAGGTCTG | |||||
| B | JPF (19) | GCGCTCAGGCTGCCGACGCAA | omp2b | 193 | 58 | 35 |
| JPR (19) | ACCAGCCATTGCGGTCGGTA | |||||
| C | P1 (9) | TGGAGGTCAGAAATGAAC | omp2 | 282 | 50 | 30 |
| P2 (9) | GAGTGCGAAACGAGCGC | |||||
| D | 26A (10) | GCCCCTGACATAACCCGCTT | bp26c | 1029 | 58 | 30 |
| 26B (10) | GAGCGTGACATTTGCCGATA |
a
Encoding an immunogenic 31-kDa outer membrane protein of B. abortus.
b
Encoding a 26-kDa outer membrane protein of B. abortus.
c
Encoding the BP26 protein of B. melitensis, also named Omp28.