TABLE 1.

C. neoformans strains tested in this study and results of immunoserotyping and molecular identification of the serotype

Strain no.	Strain name	Isolationa		Immunologic serotyping		Molecular identification
		Origin	Site	Methodb	Result	REA patternc	Fragment size(s) (bp)d
1	H99	United States	CSF	Commercial kit	A	A	394
2	IHEM 11752	Congo	CSF	Commercial kit	A	A	400
3	IHEM 11753	Congo	CSF	Commercial kit	A	A	394
4	IHEM 11756	Congo	CSF	Commercial kit	A	A	394
5	IHEM 11757	Congo	CSF	Commercial kit	A	A	394
6	IHEM 11876	Congo	CSF	Commercial kit	A	A	394
7	IHEM 11877	Congo	CSF	Commercial kit	A	A	394
8	IHEM 11880	Congo	CSF	Commercial kit	A	A	394
9	IHEM 11882	Congo	CSF	Commercial kit	A	A	394
10	Tou	Mali	CSF	Homemade Ab	A	A	394
11	Fat	Congo	CSF	Homemade Ab	A	A	400
12	coc 119	Cochin, India	CSF	Homemade Ab	A	A	394
13	333	Gabon	CSF	Homemade Ab	A	A	394
14	2598 P	Ivory Coast	CSF	Homemade Ab	A	A	394
15	g 57497	Gabon	CSF	Homemade Ab	A	A	394
16	Oug 1	Uganda	CSF	Homemade Ab	A	A	394
17	PP38	Cambodia	CSF	Homemade Ab	A	A	394
18	RV66254	Rwanda	CSF	Homemade Ab	A	A	394
19	3/768	Congo	CSF	Homemade Ab	A	A	394
20	Lim	France	CSF	Homemade Ab	D	A	394
21	C3	Cambodia	CSF	Homemade Ab	A	A	394
22	C6	Cambodia	CSF	Homemade Ab	A	A	394
23	C7	Cambodia	CSF	Homemade Ab	A	A	394
24	Oug 7	Uganda	CSF	Homemade Ab	A	A	394
25	PP29	Cambodia	CSF	Homemade Ab	A	A	394
26	RV68535	Rwanda	CSF	Homemade Ab	A	A	394
27	RV68528	Rwanda	CSF	Homemade Ab	A	A	394
28	Tnn Ci	France	CSF	Homemade Ab	A	A	394
29	g 59546	Gabon	CSF	Homemade Ab	A	A	394
30	RV66254	Rwanda	CSF	Homemade Ab	A	A	394
31	C57	Cambodia	CSF	Homemade Ab	A	A	394
32	C68	Cambodia	CSF	Homemade Ab	A	A	394
33	Oug 3	Uganda	CSF	Homemade Ab	AD	A	394
34	Oug 9	Uganda	CSF	Homemade Ab	A	A	394
35	PP43	Cambodia	CSF	Homemade Ab	A	A	394
36	241	Gabon	CSF	Homemade Ab	A	A	394
37	Tnn Mb	France	Blood	mAb IF	A	A	394
38	Tnn Da	France	Blood	mAb IF	A	A	394
39	Tnn Mo	France	CSF	mAb IF	A	A	394
40	Bel	Belgium	CSF	Commercial kit	A	A	394
41	IHEM 4157	Belgium	NS	Commercial kit	AD	AD	382, 394
42	IHEM 13877	Belgium	CSF	Commercial kit	AD	AD	382, 394
43	IHEM 14440	Belgium	CSF	Commercial kit	AD	AD	382, 394
44	IHEM 16079	Belgium	Voice prosthesis	Commercial kit	AD	AD	382, 394
45	IHEM 16513	Peru	CSF	Commercial kit	AD	AD	382, 394
46	IHEM 17861	Peru	CSF	Commercial kit	AD	AD	382, 394
47	RV 66423	Rwanda	CSF	Homemade Ab	A	D	382
48	JEC21	NS	NS	Commercial kit	D	D	382
49	B 3501 A	NS	NS	Commercial kit	D	D	382
50	NIH D52	United States	CSF	Commercial kit	D	D	382
51	IHEM 15038	Peru	NS	Commercial kit	D	D	382
52	IHEM 18620	Belgium	Skin	Commercial kit	D	D	382
53	IHEM 16711	Belgium	Skin	Commercial kit	D	D	382
54	IHEM 4158	Italy	NS	Commercial kit	D	D	382
55	AKD 30	Ivory Coast	CSF	Homemade Ab	A	D	380
56	T 6073	France	CSF	Homemade Ab	D	D	382
57	2815	Cambodia	CSF	Homemade Ab	A	D	382
58	Tnn Re	France	BAL fluid	mAb IF	D	D	382
59	JLP34	France	CSF	mAb IF	D	D	382
60	JLP23	France	Urine	mAb IF	D	D	382
61	IHEM 11758	Congo	CSF	Commercial kit	D	D	380
62	C9	Cambodia	CSF	Homemade Ab	A	D	382
63	IHEM 14934	Brazil	NS	Commercial kit	B	B	373
64	IHEM 4161e	United States	Sputum	Commercial kit	B	B	373
65	IHEM 11796e	Zaire	CSF	Commercial kit	B	B	373
66	IHEM 4170	Malaysia	NS	Commercial kit	B	B	373
67	IHEM 4164	China	CSF	Commercial kit	B	B	371
68	WM 276	Australia	Environment	Commercial kit	B	B	371
69	VanR265	Canada	BWF	Commercial kit	B	B	380
70	RV68522	Rwanda	CSF	Homemade Ab	D	B	371
71	IHEM 10079	NS	CSF	Commercial kit	C	C	366
72	IHEM 4159e	United States	CSF	Commercial kit	C	C	366

^aNS, not specified; CSF, cerebrospinal fluid; BAL, bronchoalveolar lavage; BWF, bronchial wash fluid; Congo, Democratic Republic of the Congo.

^bThe commercial kit used was the Crypto Check kit (Iatron Labs, Tokyo, Japan). Homemade Ab, agglutination with homemade antisera (9); mAb IF, immunofluorescence with monoclonal antibody specific for CPS (3).

^cREA pattern deduced from in silico analysis (Table 2).

^dPCR fragments are supposed to be 8 bases longer than the sequences due to a 7-base tail added to the reverse primer used to promote the addition of an extra A to the amplified fragment.

^eType strain.