TABLE 2.
Expected sizes (bp) of CAP59 gene fragments from different serotypes before and after restriction with BsmFI, HpaII, and AgeI enzymes
| Assay or fragment | Expected size(s) (bp) for serotypea:
|
|||
|---|---|---|---|---|
| A | B | C | D | |
| Native amplicon | 387 | 373 | 359 | 375 |
| REA with BsmFI | 206, 191 | NRS | NRS | NRS |
| REA with HpaII | NRS | 270, 101 | 256, 101 | 211, 162 |
| REA with AgeI | NRS | 269, 100 | NRS | NRS |
a
NRS, no restriction site; serotype A, strain H99 (GenBank accession number AF337639); serotype B, strain R265 (http://www.broad.mit.edu/annotation/genome/cryptococcus_neoformans_b/Assembly.html); serotype C, strain IHEM 4159 (sequence obtained in the present study); serotype D, JEC21 strain (GenBank accession number AE017341).