FIG. 2.

Efficiency of CMV detection by real-time PCR with a filter disk in the reaction mixture. Purified CMV samples were diluted in CMV-negative urine. Three microliters of the dilutions containing 5 × 10⁵ to 5 × 10¹ CMV genome copies per reaction mixture was added directly to 50 μl of the PCR mixture (“straight”). Three microliters of the dilutions was also applied to filter disks of 3 mm in diameter. The disks were rinsed with water and then added directly to a reaction plate for real-time PCR (“on-disc”). The x axis indicates the quantity of CMV virions added to each reaction mixture, and the y axis indicates CMV copy numbers measured by the real-time PCR. The experiment was done in triplicate.