TABLE 1.
Yeast isolates identified at the United Kingdom MRL from March 2004 through March 2006a
| Organismb | Total | % Total | AUX (no. of isolates) | Code (no. of isolates) | API 20C (no. of isolates) | Molecular IDc (no. of isolates) | % Molecular ID |
|---|---|---|---|---|---|---|---|
| Candida albicans | 1,192 (germ tube = 1,126) | 39.3 | 56 | 7145207 (30) | 3 | 7 | 0.6 |
| 7144207 (15) | |||||||
| 7141207 (4), PCRd; 7144007 (2); 7145205 (3); 7144204 (2) | |||||||
| Candida glabrata | 659 | 21.7 | 649 | 1040004 (649) | 4 | 6 | 0.9 |
| Candida parapsilosis | 430 | 14.2 | 415 | 7143105 (134); 7145105 (124); 7147105 (120); 7146105 (10); 7102105 (10); 7142105 (8) | 12 | 3 | 0.7 |
| Candida tropicalis | 156 | 5.2 | 154 | 7147005 (154) | 2 | 0 | |
| Candida lusitaniae | 99 | 3.3 | 45 | 7067005 (17); 7062005 (11); 7167005 (12); 7163005 (3); 7162005 (2) | 35 | 19 | 19.2 |
| Candida krusei | 74 | 2.4 | 70 | 1000005 (70) | 4 | 5.4 | |
| Cryptococcus neoformans | 64 | 2.1 | 61 | 7577444 (15); 7557444 (10); 7576444 (6); 7157444 (5); 7572544 (4); 7577544 (4) | 1 | 3e | 4.7 |
| Saccharomyces cerevisiae | 60 | 2.0 | 29 | 7540004 (7); 7140004 (6); 7500004 (6); 7040004 (5); 7540004 (1); 7542004 (1) | 27 | 4 | 6.7 |
| Candida guilliermondii | 53 | 1.7 | 25 | 7567105 (25) | 11 | 19* | 35.8 |
| Malassezia pachydermatis | 36 (34 direct ID) | 1.2 | NAh | NA | 2 | 5.5 | |
| Candida dubliniensis | 33 (germ tube = 15) | 1.1 | 30 | 7141207 (22), PCR; 7141007 (2); 7141205 (2); 7143207 (1); 7146607 (1); 7140207 (1) | 3 | 9.1 | |
| Trichosporon sp. | 23 | 0.8 | 17 | 7364325 (5); 7777325 (5); 7767325 (2); 3364325 (2); 7374325 (1); 7366325 (1) | 2 | 4 | 17.4 |
| Rhodotorula sp. | 19 | 0.7 | NA | NA | 19 | 0 | |
| Candida inconspicua | 17 | 0.6 | 15 | 1000004 (15) | 5* | 29.4 | |
| Candida kefyr | 15 | 0.5 | 5 | 5700005 (5) | 6 | 4 | 26.7 |
| Candida famata | 12 | 0.4 | 7 | 7567104 (7)f | 2 | 3 | 25.0 |
| Candida sp. nov. | 11 | 0.4 | 0 | NA | 11 | 100 | |
| Geotrichum sp. | 10 | 0.3 | 5 | 1000025 (4) | 2 | 3 | 30.0 |
| 1104021 (1) | |||||||
| Candida pelliculosa | 8 | 0.3 | 0 | NA | 6 | 2 | 25.0 |
| Candida fabianii | 5 | 0.2 | 0 | NA | 5g | 100 | |
| Candida lipolytica | 5 | 0.2 | 2 | 1100005 (2) | 3 | 60.0 | |
| Candida blankii | 4 | 0.1 | 0 | NA | 4 | 100 | |
| Candida utilis | 4 | 0.1 | 0 | NA | 3 | 1 | 25.0 |
| Cryptococcus albidus | 4 | 0.1 | 4 | 7776140 (2); 7676140 (1); 7066140 (1) | 0 | ||
| Candida rugosa | 3 | 0.1 | 0 | NA | 2 | 1 | 33.3 |
| Candida norvegensis | 3 | 0.1 | 0 | NA | 3 | 100 | |
| Candida pararugosa | 3 | 0.1 | 0 | NA | 3 | 100 | |
| Candida catenulata | 3 | 0.1 | 0 | NA | 3 | 100 | |
| Kloeckera sp. | 3 | 0.1 | 0 | NA | 1 | 2 | 66.7 |
| Saccharomyces elongasporus | 2 | 0 | NA | 2 | 100 | ||
| Candida zeylanoides | 2 | 0.3 | 0 | NA | 2 | 100 | |
| Candida eremophila | 2 | 0 | NA | 2 | 100 | ||
| Candida lambica | 2 | 0 | NA | 2 | 100 | ||
| Total | 3,033 | 138 | 153 | 5.0 |
Figures are given for the total number of isolates received for each species, the percentage of total isolates represented by each species, and the number of isolates identified by germ tube (C. albicans and C. dubliniensis only), AUXACOLOR2 (AUX; with the most frequent AUXACOLOR2 codes obtained), API 20C, and D1D2 sequencing (molecular ID, with the percentage of isolates of each species that required sequence analysis) analyses.
The following 17 organisms (0.5% of all isolates) were single isolates whose identification required molecular approaches: Candida ciferrii, Candida boidinii, Candida palmioleophila, Candida freyschussii, Kazachstania pintolopesii, Candida magnoliae, Candida viswanathii, Metschnikowia pulcherrima, Pichia onychis, Sporobolomyces sp., Zygosaccharomyces lentus, Issatchenkia terricola, Candida haemulonii, Candida pseudointermedia, Candida pseudoglaebosa, Sporopachydermia cereana, and Kazachstania bovina. Candida ciferrii, Candida boidinii, and Candida magnoliae were identified as part of quality control programs and were not isolates from clinical cases. Sequencing did not reliably resolve the Trichosporon, Kloeckera, and Geotrichum isolates to the species level.
Proportions of the molecular identifications indicated with an asterisk were used to confirm AUXACOLOR2 presumptive identities.
Four isolates of C. albicans gave AUXACOLOR2 profiles consistent with C. dubliniensis but were confirmed as C. albicans by intron-specific PCR (see the text).
C. neoformans isolates were identified by sequencing of the ITS1 region.
The published AUXACOLOR2 code of 7567100 for C. famata is incorrect and should read 7567104.
C. fabianii and C. veronae were initially reported to be indistinguishable on the basis of D1D2 sequencing (13).
NA, not applicable.