. 2007 Mar 14;45(5):1624–1627. doi: 10.1128/JCM.02316-06

TABLE 2.

Detection of P. malariae and P. ovale by different PCR-based protocols

No. of samples by microscopy^a	No. of samples detected by Rt-PCR with^b^,^c:			No. of samples detected by nested PCR^c^,^d
No. of samples by microscopy^a	2004R, P. ovale	2004R, P. malariae	2004P, P. ovale	No. of samples detected by nested PCR^c^,^d
9 Pf	1	0	1	6 Pf
				1 Po
				1 Pf + Po
				1 Pf + Pm + Po
12 Pv	6	1	5	5 Pv
				1 Pm
				6 Po
3 Pm	0	3	0	3 Pm
7 Po	7	0	3	7 Po
1 Pv or Po	1	0	0	1 Po
2 Plasmodium sp.	0	1	0	1 Pm
				1 Pf + Pm + Po
73 negative	1	1	1	1 Pm
				2 Po
				70 negative
3 Pm and 7 or 8 Po	16 Po	6 Pm	10 Po	8 Pm and 20 Po

Diagnosis made on admission by microscopic examination of Giemsa-stained thin blood smears for the set of 107 patients included in the analyses. Pf, P. falciparum; Pv, P. vivax; Pm, P. malariae; Po, P. ovale.

The Rt-PCRs with primers 2004R (13) and 2004P (15) were performed as described previously.

Five microliters of purified template DNA was used for the amplification assays.

For nested PCR with primer NP-2002, following a primary reaction with the genus-specific primer set rPLU1-rPLU5, detection of P. falciparum (Pf), P. vivax (Pv), and P. malariae (Pm) was carried out with primers rFAL1-rFAL2, rVIV1-rVIV2, rOVA1-rPLU2, and rMAL1-rMAL2 (19); for nested PCR with primer NP-2005, the same primary reaction described for the nested PCR with primer NP-2002 was performed, but the detection of P. ovale (Po) was achieved by using the two pairs of P. ovale-specific primers: rOVA1-rOVA2 (20, 21) and rOVA1v-rOVA2v (rOVA1v, 5′-ATCTCCTTTACTTTTTGTACTGGAGA-3′; rOVA2v, 5′-GGAAAAGGACACTATAATGTATCCTAATA-3′).