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Standard curves generated by multiplex real-time PCR. Serial dilutions of each viral standard ranging from 5 to 5 × 106 copies per reaction were used to generate the standard curves. The cycle of threshold values that corresponded to the PCR cycle number was plotted against the copy number of each viral standard. Circles, EBV DNA standard plasmid; triangles, CMV DNA standard plasmid; squares, HHV-6 standard plasmid.
