FIG. 3.

(A) Measurement of PIA/PNAG immunoreactivity in four representative wild-type (WT) MSSA clinical isolates and their corresponding Δica mutants grown in BHI medium supplemented with 4% NaCl. (B) Comparison of biofilm regulation in four representative WT MRSA clinical isolates and their corresponding Δica mutants. (C) Comparison of biofilm regulation in four WT MRSA isolates and their corresponding Δica mutants. Biofilm formation was analyzed by growing triplicate cultures in tissue culture-treated 96-well plates in BHI medium or in BHI medium supplemented with 4% NaCl or 1% glucose, followed by staining with crystal violet. Photographs of representative biofilm plates are shown.