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. 2007 Feb 28;45(5):1379–1388. doi: 10.1128/JCM.02280-06

FIG. 4.

FIG. 4.

Susceptibility of glucose-induced MRSA biofilms (left) and NaCl-induced MSSA biofilms (right) to treatment with sodium metaperiodate and proteinase K. MRSA biofilms were grown in BHI medium supplemented with 1% glucose, and MSSA biofilms were grown in BHI medium supplemented with 4% NaCl for 24 h. Each strain was grown in four individual wells. The laboratory strain RN4220 (wells 2A to 2D in each plate) was used a positive control for NaCl-induced biofilm formation in MSSA. The MRSA clinical isolate BH1CC (wells 3A to 3D in each plate) was used a positive control for glucose-induced biofilm formation in MRSA. The 15 clinical MSSA isolates used in the experiment were BH6(2005) (wells 5A to 5D), BH49(2004) (wells 6A to 6D), BH51(2004) (wells 6E to 6H), BH37(2004) (wells 7A to 7D), BH48(2004) (wells 7E to 7H), BH21(2004) (wells 8A to 8D), BH29(2004) (wells 8E to 8H), BH10(2004) (wells 9A to 9D), BH18(2002) (wells 9E to 9H), BH23(2003) (wells 10A to 10D), BH27(2003) (wells 10E to 10H), BH11(2003) (wells 11A to 11D), BH17(2003) (wells 11E to 11H), BH2(2003) (wells 12A to 12D), and BH8(2003) (wells 12E to 12D). The 14 clinical MRSA isolates used in this experiment were BH26(2004) (wells 5A to 5D), BH17(2005) (wells 5E to 5D), BH4(2004) (wells 6A to 6D), BH8(2004) (wells 6E to 6H), BH23(2003) (wells 7A to 7D), BH30(2004) (wells 7E to 7H), BH10(2003) (wells 8A to 8D), BH11b(2003) (wells 8E to 8H), BH14(2002) (wells 9A to 9D), BH16(2002) (wells 9E to 9H), BH10(2002) (wells 10A to 10D), BH12(2002) (wells 10E to 10H), BH2(2002) (wells 11A to 11D), and BH6(2002) (wells 11E to 11H).