FIG. 3.

Visual detection of LAMP products. (A) The BKV LAMP products were detected after addition of SYBR green I dye. The tube with a positive reaction (tube 1) shows a color change to yellowish green, which can be distinguished from the reddish orange color of a negative reaction. (B) The same positive sample, when visualized under UV transillumination, shows a bright green fluorescence in tube 1 (positive reaction) compared to what is observed for tube 2 (negative reaction). (C) A portable blue light illuminator that can be used in a clinic setting for transillumination of LAMP products. A 0.5-ml PCR tube is placed on it to show the relative size of the transilluminator.