TABLE 1.
LAMP primers
| Primer used for BKV LAMP | Sequence | No. of bases |
|---|---|---|
| Outer forward and backward | ||
| BKVTF3 | GGACCCACCATTGCAGAGTTT (outer forward primer) | 21 |
| BKVTB3 | TCTTTGCCCAGATACCCTGT (outer primer) | 20 |
| Inner forward and backwarda | ||
| BKVTBIP (B1 + B2c) | TTTAAATGYCTTAATCTWAGCTGAGGAWTGGCCTATTTGTTCCA | 44 |
| BKVTFIP (F1c + F2) | TTTTAAGAAAAGAGCCCTTGGTTAGGTCTAAGCCAAACCACTGT | 44 |
| Loop forward and backward | ||
| BKVTLoop F | GATTGCTACTGCATTGACTGCT | 22 |
| BKVTLoop B | ATGCAAGGGCAGTGCAC | 17 |
a
The F1c and B2c sequences are complementary and reverse to the F1 and B2 regions shown in Fig. 1 and are shown as underlined. The BKVTBIP primer had two degenerate bases in three positions (W = A/T and Y = C/T).