TABLE 2.
Results of RT-PCR and culture for various specimens from the patient
Specimen | Conventional RT-PCR resulta | Real-time RT-PCR resultb | Culture resultc |
---|---|---|---|
Serum sample | + | Undet | C1V1 |
NPS swab (first swab) | Undetd | Undet | Undet |
NPS swab (second swab) | Undet | Undet | Undet |
Nasal swab | + | 36.4 | C1V2 |
Pharyngeal swab | + | 39.5 | Undet |
A + sign indicates that a band of the expected size was obtained in the conventional RT-PCR assay.
The numbers are the threshold cycle values obtained in the reaction. In this particular assay, values below 45 are positive.
C1V1 is CPE first observed on or after one C6/36 cell passage and one Vero cell passage, and C1V2 is after one C6/36 cell passage and two Vero cell passages.
Undet, undetected.