FIG. 6.
The lack of PKR increases viral mRNA translation during influenza virus infection. PKR KO and WT MEFs were mock infected or infected with the WSN strain of influenza virus at an MOI of 1 PFU/cell. (A) Cells were labeled with [35S]methionine for 30 min at the indicated times p.i. Cells were lysed, and labeled proteins were analyzed by SDS-10% PAGE and autoradiography. The positions of the major viral proteins NP, M1, and NS1 are indicated. (B) Densitometry analysis for three independent experiments. The density of the NP band was normalized to the density of the entire lane, with each bar representing the mean ± standard deviation.