FIG. 8.

The infectivity of BTV-1 ssRNA is sensitive to RNase A. BSR monolayers were transfected with RNase A digests of either 2 μg (307 fmol) BTV-1 ssRNA (A) or 20 ng BTV-1 cores (B) and overlaid with agarose, as described in Materials and Methods. The quantities of RNase A used were as follows: wells 1, no RNase A; wells 2, 100 ag; wells 3, 1 fg; wells 4, 10 fg; wells 5, 100 fg; wells 6, 1 pg; wells 7, 10 pg; wells 8, 100 pg; wells 9, 1 ng; wells 10, 10 ng; wells 11, 100 ng; wells 12, 1 μg. The monolayers were fixed and stained with crystal violet 3 days after transfection (A) or 2 days after transfection (B).