FIG. 5.

Defining the RNA binding domain in hUL47. (A) Line drawing of the hUL47 N-terminal GST fusion proteins expressed and purified from E. coli. (B and C) Approximately equivalent amounts of each GST fusion protein, together with GST alone, were subjected to SDS-PAGE followed by Coomassie blue staining or transfer to nitrocellulose membrane. Northwestern (N-Western) blotting was carried out using an RNA probe made by purifying total RNA from uninfected cells labeled for 4 h with [³²P]orthophosphate. Molecular mass markers (in kilodaltons) are noted at the left of blots.