FIG. 6.

Defining the NLS in hUL47. (A) COS-1 cells were transfected with plasmids expressing either GFP-hUL47 (wild type [Wt]) or GFP-hUL47 with arginine boxes 2 and 3 mutated to glycines (ΔR²⁺³). Transfected cells were examined live 20 h after transfection. (B) Line drawing of the N-terminal regions of hUL47 fused to the β-galactosidase reporter. (C) COS-1 cells were transfected with plasmids expressing β-galactosidase alone or the fusions shown in panel B. Forty hours after transfection, the cells were fixed and processed for immunofluorescence by use of an anti-β-galactosidase antibody.