FIG. 7.

Direct enumeration of M2_82-90 or M2_127-135 epitope-specific CD8⁺ T lymphocytes. Groups of BALB/c mice were infected twice with RSV wild-type strain A2. Splenocytes (D, E, and F) were harvested 8 days following the second infection and tested by flow cytometric analysis after staining with the M2_82-90 tetramer (B and E) or M2_127-135 tetramer (C and F). Splenocytes from naïve mice were used as negative controls (A, B, and C). Dot plots illustrate results for total CD3⁺ lymphocytes as well as 50,000 CD8⁺ T lymphocytes. Numbers shown in the rectangular gate correspond to the percentage of CD8⁺ lymphocytes stained by the respective tetramer (tet). Data represent the results of a single experiment, which was one of five with similar results.