. 2006 Dec 20;81(5):2349–2358. doi: 10.1128/JVI.01910-06

TABLE 2.

Truncated peptide synthesis strategy to identify the optimal subdominant epitope within the M2_121-139 region

Category	Peptide^a	Protein region (mutation)	Peptide sequence^b
Control	36	M2_141-155	`NNKQTIHLLKRLPAD`
Subdominant M2 epitope	31	M2_121-135	`NSPKIRVYNTVISYI`
	32	M2_125-139	`IRVYNYVISYIESNR`
	A	M2_125-135	`IRVYNTVISYI`
	B	M2_121-134	`IRVYNTVISY`
	C	M2_121-133	`IRVYNTVIS`
	D	M2_121-132	`IRVYNTVI`
	E	M2_126-135	`RVYNTVISYI`
	F	M2_127-135	`VYNTVISYI`
	G	M2_128-135	`YNTVISYI`
	H	M2_127-135 (Y128R)	`VRNTVISYI`
Dominant M2 epitope	20	M2_77-91	`VGVLESYIGSINNIT`
	21	M2_81-95	`ESYIGSINNITKQSA`
	I	M2_82-90	`SYIGSINNI`

The original 15-amino-acid peptides used for screening were numbered according to their relative positions from the N terminus (1 through 46). The truncated and variant peptides were assigned letter designations.

The regions of peptides containing the optimal predicted nine-amino-acid epitope are underlined. Predicted anchor residues at position 2 or 9 are shown in bold. A mutated residue at position 2 is shown in italics.