FIG. 6.

ZO-1 localizes to cell-cell boundaries in infected MDCK-1 cells. (A) Subconfluent cell layers of MDCK-1 in chamber slides were either infected with CCHFV (top row) or mock infected (bottom row) and fixed 48 hpi. CCHFV NP is shown in red, and ZO-1 is shown in green. (B) Confluent cells were mock treated or treated with PMA (100 nM) for 1 h. After fixation, cells were stained for ZO-1 as described in Materials and Methods.