FIG. 5.

Conditioned media from TIME cells infected with KSHV for 24 h activates STAT3. (A) Immunoblot analysis of extracts from uninfected TIME cells treated with 24 hpi conditioned media from mock-infected, UV-inactivated KSHV, and KSHV-infected TIME cells. Only conditioned media from wild-type KSHV infections were able to activate STAT3 in uninfected TIME cells. (B) Conditioned medium from KSHV-infected TIME cells was harvested 24 hpi and used to treat fresh TIME cells over a time course. Immunoblot analysis for pSTAT3 Y705 shows that conditioned medium from TIME cells at 24 hpi induces only transient STAT3 activation (lasting only 90 min) in the absence of KSHV infection.