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. 2006 Dec 6;81(5):2449–2458. doi: 10.1128/JVI.01769-06

FIG. 6.

FIG. 6.

The JAK2 inhibitor AG490 reduces STAT3 activation in KSHV infections at 24 hpi. (A) Immunoblot of extracts from KSHV-infected TIME cells (24 hpi) treated with the indicated kinase inhibitors were probed with pSTAT3 Y705 or β-actin antibodies. Only the JAK2 inhibitor AG490 reduced phospho-STAT3 levels in a dose-dependent fashion. The JAK3 inhibitor WHI-PI3I and the src family kinase inhibitor SU6656 had no effect on STAT3 phosphorylation. (B) Extracts from wild-type KSHV infections performed in the presence of AG490 or DMSO were subjected to DNA-binding assays for STAT3-specific sequences as described in Fig. 4. The DNA-binding activity of STAT3 in KSHV-infected TIME cells was inhibited by the JAK2 inhibitor AG490.