TABLE 3.
Coreceptor tropism and V3 loop amino acid sequences of pre- and postpassage SF162
| Virus, drug, and passage no. | Coreceptor tropisma | gp120 V3 loop sequenceb |
|---|---|---|
| SF162, start | R5 | CTRPNNNTRKSITIGPGRAFYATGDIIGDIRQAHC |
| SF162, control | ||
| 4 | DM | -------------r----v---------------- |
| 6 | DM | -------------r----v---------------- |
| 12 | DM | -------------r----v---------------- |
| SF162, maraviroc | ||
| 4 | DM | -------------R-------t------------- |
| 6 | DM | -------------R----v--T------------- |
| 12 | DM | -------------R----V--T------------- |
a
Tropism was assigned phenotypically by using the PhenoSense HIV Entry assay. R5, CCR5 tropic; DM, dual or mixed tropic.
b
Sequences were obtained by population sequencing. Amino acid positions are numbered according to HXB2. Changes shown in lowercase indicate positions where a mixture was observed between the starting sequence and the mutated sequence. Amino acids 309 and 316 are underlined in the topmost sequence.