TABLE 5.

Accumulation of mutations in gp120 during passage of CC1/85 in increasing concentrations of maraviroc

Condition	Mutation in gp120a
	Domain V2	Domain V3			Domain C3	Domain V4
	Amino acid 163	Amino acid 316	Amino acid 319	Amino acid 323b	Amino acid 355	Amino acid 405
CC1/85, start	T	A	A ≫ Tc	I ≫ Vd	N ≫ P> K = Ye	S ≫ A > T = E
Passage 5 (16)f	T	A	A	I	N > P	S
Passage 9 (32)	T > K	A	A	V	Y > N > S	S ≫ A
Passage 13 (128)	K > T	T > S	A	V	Y	A
Passage 15 (2,000)	K	T ≫ S	A > S	V	Y	A ≫ V
Passage 16 (4,000)	K	T ≫ S	A > S	V	Y	A

^aMutations were identified by sequencing the entire gp160 region of 36 envelope clones amplified from the start virus and 12 envelope clones amplified from the five other time points. Amino acid positions are numbered according to HXB2. Bold font indicates where the majority population carries a change from the prepassage virus.

^bThis could be considered position 322 according to the placement of an insertion in the alignment.

^cA319T was seen in 4/36 clones.

^dI323V was seen in 1/36 clones.

^eN355Y was seen in 1/36 clones.

^fThe concentration [nanomolar] of maraviroc in the selection culture is in parentheses.