FIG. 2.

Models of the tomogram illustrated in Fig. 1. A. Model created by computationally fitting surfaces to densities in the tomogram, representing the capsid surface (purple) and the thickened region of the INM (green). Bridging rods (yellow) were segmented by manual tracing in sequential 1-nm sections. B. Similar to panel A, but the INM is made translucent to better illustrate the attachments of the bridging rods. C. Side view of a model created by manual tracing and surface rendering of the resulting contours. The inner and outer surfaces of the capsid (purple) and the INM (green) are shown, with the bridging rods (yellow) connecting the outer surface of the capsid with the inner surface of the thickened region of the INM. D. Same as panel A, but viewed from the cytoplasmic side to show the orientation of the bridging rods relative to the capsid surface. The inner protein sphere or scaffold does not appear in the model.