FIG. 5.

Heterologous TM constructs. (A) Partial amino acid sequences of VACV A9 and B5 and of VSV G, with their TMs shown in bold. (B) Synthesis of B5 and VSV G TM polypeptides or of A9 with the B5 or VSV G TM. HeLa cells were infected with vA9i and transfected with the indicated plasmids. Sixteen hours after infection, cells were labeled with [³⁵S]methionine-cysteine. Cleared lysates were immunoprecipitated with anti-HA MAb, and bound proteins were analyzed by SDS-polyacrylamide gel electrophoresis and autoradiography.