FIG. 1.

Sample data from 10-color flow cytometry of CD4⁺ T cells specific for a panel of antigens. A. Typical responses to the indicated antigens with regard to IL-2 and IFN-γ production. In the second two rows, total IFN-γ-, TNF-α-, and IL-2-producing cells are shown (orange) on the background of all other CD4⁺ T cells (gray). B. Sample flow cytometry of cytokine production and surface phenotype during an interruption of therapy.