FIG. 4.

Proliferation of off-therapy CD4⁺ T cells to Gag peptides is rescued by coculture with autologous, on-therapy PBMC. A. Proliferation of on-therapy (left panel) and off-therapy (right panel) cells to medium and to HIV p24 antigen. CFSE-labeled cells were cultured with unlabeled cells from the same or opposite time points. A gating strategy was utilized to subtract the unlabeled cells from the culture after 6 days. B. Summary of data on proliferative responses to HIV p24 antigen and Gag peptide pools of off-therapy cells cultured with unlabeled off-therapy or on-therapy PBMC. The median response is shown in boldface.