FIG. 5.

Alterations in Gag-specific CD4⁺ T-cell cytokine production that occur during interruption of antiretroviral therapy are intrinsic to the T cells and are not rescued by culture with cells from the on-therapy time point. PBMC from on-therapy and off-therapy time points were cultured alone or mixed with autologous cells from the opposite time point. CFSE labeling of cells from one of the time points allowed the two populations of cells to be distinguished. Mixing cells from on- and off-therapy time points did not alter the frequency or proportions of cytokine-producing cells. B. Summary of data for eight patients. The median response is shown in boldface.