FIG. 4.

Analysis of viral protein synthesis, type I IFN production, and DC activation by UV-treated RRV. (A) mDC cultures were either mock infected (M) or treated with RRV, UV′-RRV, or UV"-RRV (MOI 5 or the equivalent amount), and lysates were prepared at 24 or 48 hpi and analyzed by Western blotting using the anti-RRV K230 serum (upper panel). The positions of VP2 and a murine protein reacting nonspecifically with the serum (*) are indicated at the right. The lysates were also probed with an anti-IRF-3 (middle panel) and an antiactin (lower panel) antibody. (B) Type I IFN production in the supernatants of mDCs harvested 24 h after treatment with RRV, UV′-RRV or UV′-RRV (MOI 5 or the equivalent amount) was measured using a bioassay and expressed as U/ml. (C) Upregulation of the CD86 activation marker on mDCs exposed to RRV, UV′-RRV, and UV"-RRV compared to those of untreated controls is shown as the percentage of increase in mean fluorescence intensity (MFI). The figure shows mean values ± SD from mDCs from three individual mice.