FIG. 8.

Type I IFN production in rotavirus-treated DC cultures generated in the presence of Flt3-L. (A) Phenotypes of bone marrow-derived DCs generated in the presence of Flt3-L. CD11c⁺ CD11b⁻ B220⁺ (pDCs) and CD11c⁺ CD11b⁺ B220⁻ (mDCs) are shown in the panels to the right. (B) Comparison of type I IFN production measured with a bioassay, an IFN-α ELISA, or an IFN-β ELISA from DC cultures generated in the presence of GM-CSF (mDCs) or Flt3-L (mixed pDCs and mDCs) 24 h after RRV stimulation. (C) Type I IFN levels in Flt3-L culture supernatants 6 h after treatment with DLP, TLP, and RRV were measured using the bioassay. The figure shows data representative of two independent experiments.