Skip to main content
. 2007 Jan 3;81(6):2980–2994. doi: 10.1128/JVI.02339-06

FIG. 4.

FIG. 4.

Replication of PSTVd is resistant to induced RNA silencing in N. benthamiana protoplasts. (A) PSTVd accumulates at similar levels in the absence or presence of differing amounts of siRNAs. The siRNA sequence corresponds to positions 194 to 215 of the PSTVd genome. (B) PSTVd accumulates at similar levels in the presence of 5 μg of different siRNAs synthesized based on srPSTVds. (C) Accumulation of GFP mRNA decreases with increasing amounts of GFP siRNAs. (D) PSTVd accumulates at similar levels in the absence or presence of differing amounts of dsRNAs. C-PSTVd, circular genomic PSTVd; L-PSTVd, linear unit-length PSTVd. RA, ratio of RNA accumulation levels obtained from three biological replicates. rRNA bands in PAGE stained with ethidium bromide served as a loading control. C-PSTVd was used for RA assessment.