FIG. 3.

Transduction of established and primary melanoma cells and of normal cells with fiber-modified adenoviruses. A human melanoma cell line (SK-MEL-28), primary human melanoma cells (PMel), primary human endothelial cells (HUVEC), primary normal human fibroblasts (NHF), and primary normal human keratinocytes (NHK) were transduced with the indicated viruses at 10⁴ (HUVEC), 2.5 × 10⁴ (NHF and NHK), 5 × 10⁴ (SK-MEL-28 cells), or 10⁵ (PMel cells) VP/cell in triplicate. At 2 days postinfection, cells were lysed, and β-galactosidase activities of the lysates were determined with a luminescence assay. Columns show mean RLU values; error bars show standard deviations. Uninfected cells were analyzed to show the background activity of β-galactosidase.