FIG. 6.
Analysis of pF5/41sIJ-RGD derivatives with two copies of the RGD peptide insert in tandem, an extended RGD-containing peptide insert, or a long shaft. (A) Cell lysates of 293T cells transfected with the indicated plasmids were analyzed for recombinant fiber expression and trimerization by gel electrophoresis and Western blotting as described in the legend to Fig. 2A. (B) Purified adenoviruses (8 × 108 VP) generated with the transfection/infection technology were analyzed by gel electrophoresis and Western blotting for recombinant fiber incorporation as described in the legend to Fig. 2B. (C) SK-MEL-28 and PMel cells were transduced with the indicated adenoviruses at 105 VP/cell in triplicate. At 2 days postinfection, cells were lysed, and β-galactosidase activities of the lysates were determined with a luminescence assay. Columns show mean RLU values; error bars show standard deviations. Uninfected cells were analyzed to show the background activity of β-galactosidase.