FIG. 6.

Daxx localization to PODs changes in response to rM51R-M virus infection. L929-EV and L929-ΔDaxx cells were mock infected or infected with rM51R-M virus. At 16 h postinfection (hpi), cells were analyzed by indirect immunofluorescence as described in Materials and Methods using antibodies specific for PML and Daxx. Bar, 10 μm. (A to L) Representative cells that illustrate the three patterns of Daxx and PML labeling observed: cells expressing Daxx in PODs, cells expressing PODs without Daxx, and cells not expressing PODs. (M) Quantification of cells expressing Daxx in PODs, cells expressing PODs without Daxx, and cells not expressing PODs. Data are expressed as percentages of total cells ± SEM for three experiments. (N) Cells were mock infected or infected with rWT virus or rM51R-M virus. At the indicated times postinfection, cell lysates were analyzed via Western blotting with antibodies for PML and β-actin. Western blots were quantitated via densitometric analysis. Levels of PML protein expression were normalized to levels of actin for each sample. Results indicate the averages of three independent experiments ± SEM (ast;P < 0.05 relative to mock-infected samples). DIC, differential interference contrast.