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. 2006 Dec 20;81(6):2564–2572. doi: 10.1128/JVI.02449-06

FIG. 2.

FIG. 2.

Characterization of recombinant MVs containing chimeric H proteins, with or without N481Y substitution. (A) Entry efficiencies of recombinant MVs in HeLa cells. The number of CIU of each virus stock was determined in HeLa and Vero/hSLAM cells, and the CIU in HeLa cells was compared with that in Vero/hSLAM cells. The CIU in Vero/hSLAM cells was set to 100%. (B) EGFP autofluorescence in MV-infected cell monolayers. B95a and HeLa cells were infected with recombinant MVs at an MOI of 0.01. Panels show representative images captured with a fluorescence microscope 2 days after infection. (C) Replication kinetics of recombinant MVs. HeLa and Jurkat cells were infected with recombinant MVs at an MOI of 0.01. At various time intervals, cells were harvested in culture medium, and CIU were determined in Vero/hSLAM cells. Average CIU for duplicate experiments are shown.