FIG. 1.

Cytotoxic effect of MTH-68/H on different cell lines. Cells (10⁴, or in the case of the undifferentiated wild-type PC12 cells, 4 × 10⁴) were cultured in 24-well tissue culture plates. Twenty-four hours after being plated, the cells were infected with different titers of MTH-68/H, as indicated. For the positive control, cells were treated with anisomycin (1 μg/ml); for the negative control, they were grown in culture medium without treatment. After 72 h of incubation, WST-1 assays were performed as described in Materials and Methods. Results for the untreated cells and the anisomycin controls are shown on the left (-) and right (A) sides of the charts, respectively. (A) p53-positive tumor cell lines; (B) p53-negative tumor cell lines; (C) nontransformed fibroblast cell lines.