FIG. 4.

In vitro growth analysis of BAC-derived RRV isolates. A single-step growth curve of four BAC-derived viral isolates (BAC isolates 1, 6, 13, and 15) was performed with primary rhesus fibroblasts infected at an MOI of 2.5, using wild-type RRV₁₇₅₇₇ as a control (Wild Type RRV). Supernatants and cells were collected at the indicated time points postinfection, and the amount of virus in each sample was assessed by plaque assay. Each time point assessment was performed in duplicate, and the average value is indicated. Error bars represent standard deviations.