Percent reduction of Gag p27+ target cells following an 8-day coculture with SIV-specific CD8+ T-cell lines at an E:T ratio of 1:10. Twenty-six epitope-specific CD8+ T-cell lines directed against seven SIV epitopes (Tat28-35SL8, Gag181-189CM9, Gag71-79GY9, Env788-795RY8, Env620-628TL9, Vif97-104WY8, and Nef159-167YY9) were used in the VSA. These CD8+ T-cell lines were derived from 10 SIV-infected Indian rhesus macaques. The black bars indicate the average reduction for each SIV-specific CD8+ T-cell response. Exact values are listed in Table 2.