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Gag181-189CM9- and Tat28-35SL8-specific CD8+ T lymphocytes were unable to suppress the replication of an SIVmac239 escape variant. (A) Amino acid sequences of the wild-type and variant epitopes located in Gag and Tat. (B) Intracellular Gag p27 staining of representative day 8 VSA results using effector and target cells at 1:10 infected with either wild-type SIVmac239 or an escape variant, SIVmac239. (C) Quantitative PCR of viral RNA copies/ml on days 4, 6, and 8 of the same assay.
