FIG. 2.

Determination of proinflammatory cytokine levels in lungs of B6/129 mice. B6/129 mice were infected with 1,000 MID₅₀ of HK/483 or HK/486 virus. Four mice from each virus-infected group were euthanized on either day 3 or day 6 p.i., and tissue homogenates were generated for analysis. IL-1β (A), TNF-α (B), IL-6 (C), and MIP-1α (D) levels were assayed by ELISA as described in Materials and Methods. Bars represent mean cytokine concentrations (in picograms per milliliter) ± SE. The horizontal lines denote constitutive levels present in uninfected B6/129 mice. *, P ≤ 0.02.