TABLE 1.

Analysis of lymphocyte populations in thymus and blood of TNFR1-deficient and wild-type control mice infected with HK/483 virus^a

Mouse	Virus	Total no. of cells per thymus samplea	Mean % of total thymocytes ± SE			No. of leukocytes/μl	% Lymphocytes in peripheral bloodc
			CD4+ CD8+	CD4+ CD8−	CD4− CD8+
B6/129	None	1.02 × 108	89.0 ± 0.6	6.5 ± 0.9	2.0 ± 0.1	7.0 × 103	66.2
B6/129	HK/483	1.26 × 107b	42.5 ± 13.7b	32.0 ± 9.8	14.8 ± 2.5b	1.1 × 103d	24.2b
TNFR1KO	None	2.21 × 108	91.0 ± 0.6	5.5 ± 0.3	1.5 ± 0.3	6.2 × 103	63.8
TNFR1KO	HK/483	1.18 × 108	63.0 ± 7.9b	18.8 ± 4.8	7.8 ± 1.9b	1.6 × 103d	44.7

^aFour mice were infected with 1,000 MID₅₀ of HK/483 virus; 6 days later, animals were euthanized for collection of blood and thymus. A single-cell suspension of thymocytes was prepared from each mouse; cells were then counted and subjected to flow cytometric analysis as described in Materials and Methods by gating of the lymphocyte population.

^bP ≤ 0.05 compared with uninfected group.

^cFour mice from each group were bled via the orbital plexus on days 0 and 6 p.i. Total peripheral white blood cell count analyses were performed using Turk's solution as previously described (59). Percentages of lymphocytes were determined by counting 100 white blood cells on fixed blood smear slides.

^dP ≤ 0.06 compared with uninfected group.