FIG. 2.

Activation of transcription factor activities by STP-A. 293T cells were transfected with 0.5 μg of pEF.IRES.puro, pEF.IRES.puro-STP-A WT, pEF.IRES.puro-STP-A Y₁₁₅F (YF) mutant, Lck, Fyn, and Src expression vectors in various combinations along with 0.5 μg of a luciferase reporter construct and 0.1 μg of a transfection control β-Gal expression construct (pGK-β-gal). After 36 h, cells were harvested, and luciferase and β-Gal values were determined and reported as x-fold increased luciferase activity divided by β-Gal activity relative to that of vector-transfected cells. Bars represent means ± standard errors of the means (SEM) for three independent experiments. WCLs used for luciferase assays were subjected to IB with anti-Myc, anti-Src, and anti-HA antibodies to detect Lck and Fyn, Src, and STP-A expression, respectively (data not shown).