FIG. 4.
Reduced T-cell cytokine production stimulated by hepatocytes extends to TNF-α and IL-2 and is not altered by the duration of T-cell activation. (A) HBc18-27 T-cell CD107a and TNF-α production measured by double staining after 5 h of incubation with EBV B cells or HepG2 cells pulsed with the indicated peptide concentrations. (B) IFN-γ, TNF-α, and IL-2 measured in the supernatant of HBc18-27 T-cell clones cocultured with EBV B cells or HepG2 cells for 5 or 24 h. Data presented are representative of at least two individual experiments.