FIG. 5.
IRF3 and Npro interact with each other. HEK 293T cells were transfected with plasmids pFLAG-IRF3 and pEAK-Npro for the expression of FLAG-tagged IRF3 and untagged Npro (A) or with pFLAG-Npro and pIRF3 for the expression of FLAG-tagged Npro and untagged IRF3 (B), either individually or mixed at a 1:1 ratio. The presence and absence of the expression plasmid are indicated by + and −, respectively. Twenty-four hours after transfection, the cells were lysed and the proteins were immunoprecipitated with anti-FLAG M2 agarose. The precipitated proteins were eluted from the agarose, separated by SDS-PAGE, and analyzed by Western blotting (WB) with rabbit anti-Npro serum, anti-IRF3 MAb 34/1, and anti-FLAG MAb as indicated.