FIG. 7.
CSFV Npro does not downregulate IRF7 expression. PK-15 cells were transfected with plasmid pFLAG-IRF7 (A) or pFLAG-IRF3 (B) for CMV promoter-driven expression of FLAG-tagged IRF7 or FLAG-tagged IRF3, respectively. Twenty-four hours after transfection, the cells were mock treated or infected with CSFV or ΔNpro CSFV at an MOI of 2 TCID50/cell. On the indicated days p.i., cells were lysed and extracts were analyzed for IRF7 (A) and IRF3 (B) expression by Western blotting using the rabbit anti-FLAG MAb. (C) The IRF7- and IRF3-specific signals from the Western blots shown in panels A and B were quantified with the Odyssey Imaging system and expressed as percentages of the strongest signal.