TABLE 1.

Sequences of primers used for mutagenesis

Primer	Sequence (5′→3′)
MHVE_Ala1 (forward)	GTTTAATTTATTCCTTACAGACGCCACAGTATGGTATGTGGGGCA
MHVE_Ala1 (reverse)	TAAAAATAATCTGCCCCACATACCATACTGTGGCGTCTGTAAGG
MHVE_Ala2 (forward)	CCTTACAGACACAGTATGGTATGCCGTGGGGCAGATTATTTTTAT
MHVE_Ala2 (reverse)	CACACTGCGAATATAAAAATAATCTGCCCCACGGCATACCATACT
MHVE_Ala3 (forward)	CAGTATGGTATGTGGGGCAGATTGCCATTTTTATATTCGCAGTG
MHVE_Ala3 (reverse)	CCATCAAACACACTGCGAATATAAAAATGGCAATCTGCCCCA
MHVE_Ala4 (forward)	GGGCAGATTATTTTTATATTCGCCGCAGTGTGTTTGATGGTC
MHVE_Ala4 (reverse)	CACAATTATGGTGACCATCAAACACACTGCGGCGAATATAAAA
MHVE_Ala5 (forward)	ATTTTTATATTCGCAGTGTGTTTGGCCATGGTCACCATAATTGTG
MHVE_Ala5 (reverse)	GCAACCACAATTATGGTGACCATGGCCAAACACACTGCG
MHVE_Ala6 (forward)	CAGTGTGTTTGATGGTCACCATAGCCATTGTGGTTGCCTTCCTTG
MHVE_Ala6 (reverse)	TGATAGACGCAAGGAAGGCAACCACAATGGCTATGGTGACCATC
MHVE_Ala7 (forward)	CCATAATTGTGGTTGCCGCCTTCCTTGCGTCTATCAAAC
MHVE_Ala7 (reverse)	GAATACAAAGTTTGATAGACGCAAGGAAGGCGGCAACC
MHVE_Ala8 (forward)	GTGGTTGCCTTCCTTGCGGCCTCTATCAAACTTTGTATTC
MHVE_Ala8 (reverse)	CCGCAAAGTTGAATACAAAGTTTGATAGAGGCCGCAAGG