Curcumin does not inhibit replication of CVB3 through MAPK pathways. HeLa cells were infected with CVB3 in the absence or presence of curcumin (10 and 30 μM). Seven hours postinfection (p.i.), the phosphorylation status of MAPKs was examined using anti-phospho-ERK1/2, anti-phospho-p38, and anti-phospho-JNK1/2 antibodies, respectively. Expression levels of total ERK1/2, p38, and JNK1/2 were examined as the loading controls. The data represent the results from two independent experiments.