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Curcumin probably does not inhibit replication of CVB3 through CKII and CSN. HeLa cells were transiently transfected with specific siRNAs to knock down the expression of CKIIα (A), CKIIα/β (B), or Jab1 (C), respectively, for 24 h. Seven hours after CVB3 infection, viral protein expression (VP1) and levels of CKIIα, CKIIβ, and Jab1 were determined by Western blotting. β-Actin was examined to verify equal loading. The data represent the results from three independent experiments.
