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. 2007 Jan 17;81(7):3142–3150. doi: 10.1128/JVI.02028-06

FIG. 5.

FIG. 5.

Curcumin promotes protein polyubiquitination and decreases free ubiquitin levels. (A) HeLa cells were treated with DMSO or curcumin (30 μM) for 5 h. Protein polyubiquitination and free ubiquitin levels were examined by Western blot analysis using a polyclonal anti-ubiquitin antibody. β-Actin was examined to verify equal loading. (B) HeLa cells were incubated with either DMSO, MG132 (5 μM), or curcumin (30 μM) for 5 h. Ubiquitin conjugates were determined as for panel A. Multiple lanes represent the results from independent experiments.